24개월간 자일리톨껌 저작에 따른 S. mutans의 치아우식증 발생과 관련된 독성 유전자 발현 억제효과
The Inhibitive effect of 24 months xylitol gum chewing on the virulence related gene expression of Streptococcus mutans
김지혜, 서영규, 이영은, 안상헌, 구효진, 최연희, 송근배,
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김지혜 ( Kim Ji-Hye ) - 경북대학교 치의학전문대학원 예방치과학교실
서영규 ( Seo Young-Gyu ) - 경북대학교 치의학전문대학원 예방치과학교실
이영은 ( Lee Young-Eun ) - 경북대학교 치의학전문대학원 예방치과학교실
안상헌 ( Ahn Sang-Hun ) - 동국대학교 의과대학 치과학교실
구효진 ( Gu Hyo-Jin ) - 경북대학교 치과대학 예방치과학교실
최연희 ( Choi Youn-Hee ) - 경북대학교 치의학전문대학원 예방치과학교실
송근배 ( Song Keun-Bae ) - 경북대학교 치의학전문대학원 예방치과학교실
KMID : 0355420090330040534
Abstract
Objective: The aim of this study was to examine the effect of chewing gum containing xylitol on inhibiting the virulence related gene expression of Streptococcus mutans (S. mutans).
Methods: The subjects voluntarily participating in a women’s oral health prevention program were divided into two groups (a control and a xylitol group), and then they were followed for 2 years. At baseline, 6, 12, 18 and 24 months whole stimulated saliva samples were collected from the subjects. The analysis of the colony count, stereomicroscopy and real-time reverse transcription polymerase chain reaction were performed to analyze the characteristics of S. mutans.
Results: The S. mutans counts decreased steadily in the xylitol group over the study period (p< 0.05). In addition, the size of the colonies isolated from the xylitol group was smaller and their rough surface changed into a smooth surface. The expression of gtfA, gtfB, gtfC, gtfD, ftf, Putative inorganic ion ABC transporter and ATP-binding protein gene were significantly reduced in the xylitol group (p<0.05). The expression of gtfA, gtfB, gtfD, ftf, Putative inorganic ion ABC transporter and the ATP-binding protein gene in the xylitol group were significantly lower than those of the control group at 6, 12, 18 and 24 months (p< 0.05).
Conclusions: These findings indicate that chewing xylitol gum over a long period may decrease the expression of the genes associated with S. mutans virulence, which can result in the reduced size and growth of S. mutans colonies.
키워드
독력관련 유전자 발현;자일리톨;Streptococcus mutans
Streptococcus mutans;virulence related gene expression;xylitol
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